Serviços

Laboratórios

Everson Alves Miranda

Prof. Everson

DEMbio - Departamento de Engenharia de Materiais e Bioprocessos

Área de Concentração:  Desenvolvimento de Processos Biotecnológicos

Telefone: (19) 3521-3918

E-mail:

Currículo Lattes (link)

 

 

Formação 

  • Engenharia Química - Universidade Federal de Minas Gerais - 1982
  • M.S. em Engenharia Agrícola - Michigan State University, EUA - 1989
  • Ph. D. em Engenharia Química - Michigan State University, EUA - 1993
  • Pós-Doutorado na École Superieur de Biotechnologie de Strasbourg
        - Université Louis Pasteur, Strasbourg, França - 1993


Áreas de Pesquisa

  • Recuperação e Purificação de Bioprodutos

  • Cristalização de proteínas


Publicações Relevantes

 

  • FARINAS, Cristiane Sanchez; Scarpelini, Larissa Matos; Miranda, Everson Alves; Bertucci Neto, Victor. Evaluation of operational parameters on the precipitation of endoglucanase and xylanase produced by solid state fermentation of Aspergillus niger. Brazilian Journal of Chemical Engineering, v. 28, p. 17-26, 2011.

  • ROBIC, G. ; FARINAS, Cristiane Sanchez ; RECH, E. L. ; MIRANDA, E. A. . Transgenic soybean seed as protein expression system: aqueous extraction of recombinant b-glucuronidase. Applied Biochemistry and Biotechnology , v. 160, p. 1157-1167, 2010.
  • ROBIC, G. ; Miranda, Everson Alves . Modeling of protein and phenolic compound removal from aqueous solutions by electrocoagulation. Biotechnology Progress (Print) , v. 26, p. 186-191, 2010.
  • WATANABE, Erika Ohta ; Popova, Ekaterina ; Miranda, Everson Alves ; MAURER, Gerd ; Filho, Pedro de Alcântara Pessôa . PHASE EQUILIBRIA OF LYSOZYME PRECIPITATION WITH THE VOLATILE SALT AMMONIUM CARBAMATE. Fluid Phase Equilibria , v. 292, p. 42-47, 2010.
  • TAMAGAWA, Rosana Emi ; MIRANDA, E. A. ; Santana, Cesar Costapinto ; Giulietti, Marco . Determination of the Binary and Ternary Phase Diagrams of (+)-/ (?)-Ketamine Using Differential Scanning Calorimetry. Journal of Chemical and Engineering Data , v. 54, p. 16-21, 2009.
  • BRESOLIN, I. T. L. ; Miranda, Everson Alves ; BUENO, S. M. A. . CROMATOGRAFIA DE AFINIDADE POR ÍONS METÁLICOS IMOBILIZADOS (IMAC) DE BIOMOLÉCULAS: ASPECTOS FUNDAMENTAIS. Química Nova (Impresso) , v. 32, p. 1288-1296, 2009.
  • BARROS, Geórgia de Oliveira Figueiredo ; TAMAGAWA, Rosana Emi ; Santana, Cesar Costapinto ; Miranda, Everson Alves . Ternary phase diagram of ketamine ((R,S)-2-(2-chlorophenyl)-2-methylaminocyclohexanone) in ethanol and preliminary studies aiming at. Brazilian Journal of Chemical Engineering , v. 26, p. 427-434, 2009.
  • TASHIMA, Alexandre Keiji ; Ottens, Marcel ; van der Wielen, Luuk A. M. ; Cyntra, Dennys E. ; Pauli, José R. ; Alcântara Pessôa Filho, Pedro de ; Miranda, Everson Alves . Precipitation of porcine insulin with carbon dioxide. Biotechnology and Bioengineering , v. 103, p. 909-919, 2009.
  • WATANABE, Erika Ohta ; Popova, Ekaterina ; Miranda, Everson Alves ; MAURER, Gerd ; Filho, Pedro de Alcântara Pessôa . PHASE EQUILIBRIA FOR SALT-INDUCED LYSOZYME PRECIPITATION: EFFECT OF SALT TYPE AND TEMPERATURE. Fluid Phase Equilibria , v. 281, p. 32-39, 2009.
  • ROBIC, G. ; Lacorte, Cristiano ; Miranda, Everson Alves . Fluorometric quantification of green fluorescent protein in tobacco leaf extracts. Analytical Biochemistry , v. 392, p. 8-11, 2009.
  • Hirata, Gisele Atsuko Medeiros ; Bernardo, André ; Miranda, Everson Alves . Crystallization of porcine insulin with carbon dioxide as acidifying agent. Powder Technology , p. 54-57, 2009.
  • PÉREZ, V. H. ; MIRANDA, E. A. ; VALENÇA, G. P. . Kinetics of gas phase hydrolysis of ethyl acetate catalyzed by immobilized lipase. Applied Biochemistry and Biotechnology , v. 136, p. 23-38, 2007.
  • FARINAS, C. S. ; LEITE, Adilson ; MIRANDA, E. A. . Recombinant human proinsulin from transgenic corn endosperm: solvent screening and extraction studies. Brazilian Journal of Chemical Engineering , v. 24, p. 315-323, 2007.
  • KAPRITCHKOFF, F. M. ; VIOTTI, A. P. ; ALLI, R. C. P. ; ZUCCOLO, M. ; PRADELLA, J. G. C. ; MAIORANO, A. E. ; MIRANDA, E. A. ; BONOMI, A. . Enzymatic recovery and purification of polyhydroxybutyrate produced by Ralstonia eutropha. Journal of Biotechnology , v. 122, p. 453-462, 2006.
  • WATANABE, Erika Ohta ; PESSOA FILHO, Pedro A ; MIRANDA, E. A. ; MOHAMED, Rahoma Sadeg . Evaluation of the use of volatile electrolyte system produced by ammonia and carbon dioxide in water for the salting-out of proteins: precipitation of porcine trypsin. Biochemical Engineering Journal , v. 30, p. 124-129, 2006.
  • ROBIC, G. ; FARINAS, Cristiane Sanchez ; RECH, E. L. ; BUENO, S. M. A. ; MIRANDA, E. A. . Downstream process engineering evaluation of transgenic soybean seeds as hosts for recombinant protein production. Biochemical Engineering Journal , v. 32, p. 7-12, 2006.
  • AQUINO, L. C. L. ; SOUSA, H. R. T. ; MIRANDA, E. A. ; VILELA, Luciano ; BUENO, S. M. A. . Evaluation of IDA-PEVA hollow fiber membrane metal ion affinity chromatography for purification of a histidine-tagged human proinsulin. Journal of Chromatography. B , v. 834, p. 68-76, 2006.
  • BERNARDO, A. ; CALMANOVICI, C. E. ; MIRANDA, E. A. . Observance of polymorphic behaviour during dissolution of insulin and lysozyme. Brazilian Journal of Chemical Engineering , Brasil, v. 22, n. x, p. 331-339, 2005.
  • AZZONI, A. R. ; FARINAS, Cristiane Sanchez ; MIRANDA, E. A. . Transgenic corn seed for recobinant protein production: relevant aspects on the aqueous extraction of native components. Journal of the Science of Food and Agriculture , v. 85, n. x, p. 609-614, 2005.
  • SERPA, Gisele ; AUGUSTO, Elisabeth Fp ; TAMASHIRO, Wirla Msc ; RIBEIRO, Mariana B ; MIRANDA, E. A. ; BUENO, Sonia Ma . Evaluation of immobilized metal membrane affinity chromatography for purification of an immunoglobulinG1 monoclonal antibody. Journal of Chromatography. B , v. 816, n. x, p. 259-268, 2005.
  • AZZONI, A. R. ; TAKAHASHI, Kátia ; WOODARD, S. ; MIRANDA, E. A. ; NIKOLOV, Z. L. . Purification of recombinant aprotinin produced in transgenic corn seed: separation from CTI utilizing ion-exchange chromatography. Brazilian Journal of Chemical Engineering , v. 22, p. 323-330, 2005.
  • FARINAS, C. S. ; LEITE, Adilson ; MIRANDA, E. A. . Aqueous extraction of recombinant human proinsulin from transgenic maize endosperm. Biotechnology Progress , v. 21, p. 1466-1471, 2005.
  • BERNARDO, A. ; CALMANOVICI, C. E. ; MIRANDA, E. A. . Induction time as an instrument to enhance comprehension of protein crystallisation. Crystal Growth & Design , Columbus, OH, EUA, v. 4, n. 4, p. 799-805, 2004.
  • FARINAS, Cristiane Sanchez ; LEITE, Adilson ; MIRANDA, E. A. . Aqueous extraction of maize endosperm: insights for recombinant protein hosts based on downstream processing. Process Biochemistry , v. 40, n. x, p. 3327-3336, 2004.
  • FARINAS, Cristiane Sanchez ; BUENO, S. M. A. ; MIRANDA, E. A. . Adsorption of glucagon and insulin onto immobilized ion affinity chromatography silica matrix. Adsorption Science & Technology , Essex, Inglaterra, v. 21, n. 10, p. 883-896, 2003.
  • AQUINO, L. C. L. ; MIRANDA, E. A. ; ROSA, P. T. V. E. ; BUENO, S. M. A. . Adsorption of human immunoglobulin G onto methacrylate and histidine-linked methacrylate. Brazilian Journal of Chemical Engineering , Campinas, v. 20, n. 3, p. 251-262, 2003.
  • VANÇAN, S. ; MIRANDA, E. A. ; BUENO, S. M. A. . IMAC of human IgG: studies with IDA-immobilized copper, nickel, zinc, and cobalt ions and different buffer systems. Process Biochemistry , v. 37, n. 6, p. 573-579, 2002.
  • PIRES, E. L. ; MIRANDA, E. A. ; VALENÇA, G. P. . Gas-phase enzymatic esterification on immobilized lipases in MCM-41molecular sieves. Applied Biochemistry and Biotechnology , v. 98-100, n. 1, p. 963-976, 2002.
  • GENARO, A. C. B. ; TAMAGAWA, Rosana Emi ; AZZONI, A. R. ; BUENO, S. M. A. ; MIRANDA, E. A. . Recovery and puridication of aprotinin from industrial insulin processing efluent by immobilized chymotripsin and negative IMAC. Process Biochemistry , v. 37, n. 12, p. 1413-1420, 2002.
  • AZZONI, A. R. ; KUSNADI, A. R. ; MIRANDA, E. A. ; NIKOLOV, Z. L. . Recombinant aprotinin from transgenic corn seed: extraction and purification studies. Biotechnology and Bioengineering , New York, EUA, v. 80, n. 3, p. 268-276, 2002.
  • TAMAGAWA, Rosana Emi ; MIRANDA, E. A. ; BERGLUND, K. A. . Raman Spectroscopic monitoring and control of aprotinin supersaturation in hanging-drop crystallization. Crystal Growth & Design , Columbus, OH, EUA, v. 2, n. 4, p. 263-267, 2002.
  • TAMAGAWA, Rosana Emi ; MIRANDA, E. A. ; BERGLUND, K. A. . Simultaneous monitoring of protein and (NH4)2SO4 concentrationin a aprotinin hanging-drop crystallization. Crystal Growth & Design , Columbus, OH, EUA, v. 2, n. 6, p. 511-514, 2002.
  • MIRANDA, E. A. ; AZZONI, A. R. . Recovery of aprotinin from industrial process effluent by affinity adsorption. Bioprocess Engineering , Alemanha, v. 21, p. 553-560, 1999.
  • TAMAGAWA, Rosana Emi ; AZZONI, A. R. ; MIRANDA, E. A. ; VIJAYALAKSHMI, M. A. . Aprotinin recovery: comparisson between bioespecific and pseudobiospecific affinity adsorptions. Brazilian Journal of Chemical Engineering , v. 16, n. 2, p. 119-127, 1999.
  • AVELINO, S. ; ROSA, P. T. V. E. ; AZZONI, A. R. ; MIRANDA, E. A. ; SANTANA, C. C. . Recovery of cellulase by HPMC-salt precipitation: analysis by statistical experimental design. Applied Biochemistry and Biotechnology , v. 77-79, p. 807-815, 1999.
  • DECKER, P. ; MIRANDA, E. A. ; MURCIA, G. ; MULLER, S. . An improved nonisotopic test to screen a large series of new inhibitor molecules of poly(ADP-ribose)polimerase activity for therapeutic application. Clinical Cancer Research , v. 5, p. 1169-1172, 1999.
  • MIRANDA, E. A. ; MURCIA, G. ; MURCIA, J. M. . Large-scale production and purification of recombinant protein from an insect cell/baculovirus system in Erlenmeyer flasks: application to the chicken poly(ADP-ribose) polymerase catalytic domain. Brazilian Journal of Medical and Biological Research , Ribeirão Preto, v. 30, n. 8, p. 923-928, 1997.
  • LUCENA, S. L. ; MIRANDA, E. A. ; SANTANA, C. C. . The effect of external reflux on the foam fractionation of proteins. Applied Biochemistry and Biotechnology , v. 57/58, p. 57-65, 1996.
  • MIRANDA, E. A. ; DANTZER, F. ; O'FARRELL, M. ; MURCIA, G. ; MURCIA, J. M. . Characterization of a gain-of-function mutant of poly(ADP-ribose) polymerase. Biochemical and Biophysical Research Communications , v. 212, n. 2, p. 317-325, 1995.
  • MIRANDA, E. A. ; BERGLUND, Kris . Development of precipitant agents for precipitation of proteins based on hydrophobic interactions. Brazilian Journal of Chemical Engineering , Campinas, v. 12, n. 1, p. 1-12, 1995.
  • MASSON, M. ; ROLLI, V. ; DANTZER, F. ; TRUCCO, C. ; SCHREIBER, V. ; FRIBOURG, S. ; MOLINETE, M. ; RUF, A. ; MIRANDA, E. A. ; NIEDERGANG, C. ; HUNTING, D. ; GOWANS, B. ; SCHULZ, G. E. ; MURCIA, J. M. ; MURCIA, G. . Poly(ADP-ribose) plymerase: structure-function relationship. Biochimie (Paris) , Paris, v. 77, p. 456-461, 1995.
  • JUNG, S. ; MIRANDA, E. A. ; MURCIA, J. M. ; NIEDERGANG, C. ; DELARUE, Marc ; SHULZ, G. ; MURCIA, G. . Crystallyzation and X-ray crystallography analysis of recombinant chicken poly (ADP-ribose) polymerase catalytic domain overproduced in Sf9 insect cells. Journal of Molecular Biology , v. 244, p. 114-116, 1994.
  • MIRANDA, E. A. ; BERGLUND, Kris . Evaluation of colunm flotation in the downstream processing of fermentation products: recovery of a genetically engineered a-amylase. Biotechnology Progress , v. 9, n. 4, p. 411-420, 1993.
  • MIRANDA, E. A. ; BERGLUND, K. A. . Recovery of Clostridium thermosulfurogenes produced b-amylase by (hydroxypropyl)methylcellulose partition. Biotechnology Progress , v. 6, n. 3, p. 214-219, 1990.